Focus on the Sample Surface

Click the Navigate icon in the Workflow Toolbar.

Click the Sample (default) button in the Focus Sample panel.

Ensure that the Auto-compensate for fluid check box is selected.
CAUTION: If the Auto-compensate for fluid box is checked and focus is performed in air, the tip will crash on engage.

Focus on the sample surface either with the Focus: Z Motor arrows or by rolling the trackball up or down while pressing the bottom-left button. This adjustment raises or lowers the vertical engage stage on which the SPM and optics are mounted.

To move long distances hold both left trackball buttons down simultaneously and roll the trackball with high speed to lock the peak speed of motion. Release these two buttons to stop the motors. Be careful when performing this in the downward direction. Focus on the sample surface using the trackball with the bottom-left button depressed.

CAUTION: Because the command Focus Surface moves the scanner vertically, be careful when making this adjustment to ensure that the tip does not hit the sample surface.

NOTE: Fluid changes the optical path length as the angle of refraction changes, therefore, you must offset the focus position in Focus Surface by 1.3 times to a point below the surface when focusing in fluid. For example, if the surface is in focus with the Z motor positioned at –1000 µm, you must move the Z motor position down until it is at –1300 µm.

Caution/Attention/Vorsicht:

Use extreme caution moving the XY stage if the sample holder has a lip that extends above the sample surface (i.e. petri dish). The SPM scanner can be destroyed if the sample stage is moved such that the probe holder crashes sideways into the lip of your sample holder. Also, check that the position of the lip of the sample holder will not interfere with the movement of the Z-stage.
Il est recommandé de déplacer avec une extréme vigilance la platine quand le support d’échantillon présente un rebord qui se trouve à une hauteur supérieure à mesurer (comme une boîte de Petri). La tête de mesure peut être endommagée si le support de pointe vient heurter le rebord du support d’échantillon lors d’un déplacement de la platine. De plus, il est impératif de vérifier que le rebord du support d’échantillon ne viendra pas perturber le mouvement vertical du moteur de la tête de mesure.
Seien Sie extrem vorsichtig mit XY-Bewegungen des Probentisches, wenn der Rand Ihrer Probenbefestigung über die Probenfläche hinausragt (wie zum Beispiel bei einer Petrischale). Der SPM-Kopf kann zerstört werden, falls der Probentisch derart bewegt wird, daß der Spitzenhalter seitlich gegen den Rand der Probenbefestigung schlägt. Versichern Sie sich außerdem, daß der Rand Ihrer Probenbefestigung bei Bewegung der Z-Verschiebeeinheit nicht im Weg ist.

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www.bruker.com

Bruker Corporation

www.brukerafmprobes.com

112 Robin Hill Rd.

nanoscaleworld.bruker-axs.com/nanoscaleworld/

Santa Barbara, CA 93117

Customer Support: (800) 873-9750

	Add fluid.
	If necessary, Readjust the Laser Alignment.
	If necessary, Adjust the Photodetector.